Write your lab report in essay format. Your report must include the following in 1-2 pages:
- Introduction
- Hypothesis
- Methods
- Results
- Conclusion
LAB SIMULATION: STEPS
Table of Contents
PHASE 1:Prepare sample for transformation
Complete the following steps:
1. Place small tip onto small micropipette. Add 10 L of E. coli to pGLO tube. Dispose of tip
2. Place small tip onto small micropipette. Add 10 L of E. coli to pGLO tube. Dispose of tip
3. Place small tip onto small micropipette. Add 10 L of pGLO plasmid DNA to pGLO tube. Dispose of tip. Return micropipette to holder
PHASE 2:Perform heat shock and bacterial recovery
Complete the following steps:
1. Place tubes into ice bath. Incubate 10 minutes
2. Move tubes to 42C water bath. Keep in bath for 50 seconds
3. Move tubes to ice bath. Incubate for 2 minutes
4. Move tubes to lab bench
5. Place big tip onto big micropipette. Add 1000 L LB broth to pGLO tube. Dispose of tip
6. Place big tip onto big micropipette. Add 1000 L LB broth to pGLO tube. Dispose of tip. Return micropipette to holder. Let tubes stand for 10 minutes
PHASE 3:Inoculate plates with pGLO
Complete the following steps:
1.Move LB plate to lab bench. Label plate pGLO
2.Place big tip onto big micropipette. Add 100 L of pGLO to LB plate. Dispose of tip. Return micropipette to holder
3.Use sterile spreader to spread sample across plate. Dispose of spreader
4.Transfer plate to incubation tray
5.Place LB/amp plate on lab bench. Label plate pGLO
6.Place big tip onto big micropipette. Add 100 L of pGLO to LB/amp plate. Dispose of tip
7.Use sterile spreader to spread sample across plate. Dispose of spreader
8.Transfer plate to incubation tray
9.Place LB/amp/ara plate on lab bench. Label plate pGLO
10.Place big tip onto big micropipette. Add 100 L of pGLO to LB/amp/ara plate. Dispose of tip
11.Use sterile spreader to spread sample across plate. Dispose of spreader
12.Transfer plate to incubation tray
PHASE 4:Inoculate plates with pGLO
Complete the following steps:
1.Place LB plate on lab bench. Label plate pGLO
2.Place big tip onto big micropipette. Add 100 L of pGLO to LB plate. Dispose of tip
3.Use sterile spreader to spread sample across plate. Dispose of spreader
4.Transfer plate to incubation tray
5.Place LB/amp plate on lab bench. Label plate pGLO
6.Place big tip onto big micropipette. Add 100 L of pGLO to LB/amp plate. Dispose of tip
7.Use sterile spreader to spread sample across plate. Dispose of spreader
8.Transfer plate to incubation tray
9.Place LB/amp/ara plate on lab bench. Label plate pGLO
10.Place big tip onto big micropipette. Add 100 L of pGLO to LB/amp/ara plate. Dispose of tip
11.Use sterile spreader to spread sample across plate. Dispose of spreader
12.Transfer plate to incubation tray
13.Select Incubate to incubate agar plates for 24 hours
PHASE 5:After 24 hours incubation
This phase has been completed.
Complete the following steps:
Record visible light observations for pGLO plates in Lab Data
Record visible light observations for pGLO plates in Lab Data
Turn on UV light
Record UV light observations for pGLO plates in Lab Data
Record UV light observations for pGLO plates in Lab Data
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