Write your lab report in essay format.  Your report must include the following in 1-2 pages:

  • Introduction
  • Hypothesis
  • Methods
  • Results
  • Conclusion

LAB SIMULATION DETAILS:

Use of immersion oil

  • The goal of microscopy is to create a magnified image of objects too small to be seen with the eye alone.
  • Brightfield microscopes use a combination of glass lenses and light to view the specimen.
  • Using an oil immersion objective lens allows for higher magnification.
  • Microscopes are often used to study bacteria, cells, and tissues.

STEPS FOR OIL IMMERSION LAB SIMULATION


1. Focus on the cell in the slide with the yellow star using 4X objective. Use coarse and fine focus knobs to focus image
2. Rotate the nosepiece to 10x. Focus image using the fine focus knob
3. Rotate the nosepiece to 40x. Focus image using the fine focus knob
4. Rotate nosepiece halfway toward 100X objective (green ring). Apply one drop of immersion oil to slide
5. Rotate 100X lens into oil drop. Use fine focus knob to focus image
6. Rotate nosepiece to blank space next to 4x objective. Using lens paper, remove immersion oil from 100X objective and microscope slide

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