Microscopy for Microbiology – Use and Function
Hands-On Labs, Inc. Version 42-0249-00-02
Lab Report Assistant
This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.
Exercise 1: Getting to Know your Compound Microscope
Data Table 1. Microscope Components.
LetterComponent NameComponent FunctionABCDEFGHIJKLMData Table 2. Total Magnification.
LensOcular MagnificationObjective MagnificationTotal MagnificationScanningLow PowerHigh PowerOil ImmersionData Table 3. Field of View.
LensTotal MagnificationField of View (mm)Field of View (µM)ScanningLow PowerHigh PowerOil ImmersionData Table 4. Letter e Viewing Results.
LensPhotographObservationsScanningLowHighOil ImmersionQuestions
A. Describe the details in the slides “Letter e” that become visible as the power changed from scanning power, to low power, to high power.
B. Why is it important to calculate the diameter of the field when first using the microscope?
Exercise 2: Viewing Prepared Microbe Slides
Data Table 5. Prepared Slide Viewing Results.
SlidePhotographTotal MagnificationAmoebaPenicilliumYeastSpirilliumBacillusCoccusQuestions
A. Using the field of view calculated in Exercise 1 for the high power lens, approximately how far across are each of the cells in the Bacteria Coccus Form slide in Data Table 5? Show your calculations.
B. Detail techniques you found helpful for focusing on the various slides in this exercise.
Exercise 3: Preparing Wet-Mount Slides
Data Table 6. Wet-Mount Viewing Results.
SlidePhotographTotal MagnificationCheek Cell SmearDental Tatar SmearQuestions
A. Describe the similarities and differences between the cheek cell wet mount and dental plaque wet mount.
B. How did the process of preparing wet-mount slides become easier as you prepared the second wet-mount slide of this exercise?
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