Video 1  Bacterial Transformation Lab Explained (Bozeman Science)
1. What is the small segment of DNA which is passed between bacteria called? What is the name of the specific DNA segment used in this lab? Are they linear or circular?
2. What are the 4 genetic components included in the plasmid for this experiment? (write it out, don’t just copy the abbreviations)
3. What is the function of Arabinose sugar in this experiment?
4. What kind of bacteria is used in this experiment?
5. Given the components of the plasmid listed in question 2, write a hypothesis predicting the effect that adding the antibiotic Ampicillin would have on the bacteria that have the plasmid.
6. What about those that do not have the plasmid?
Video 2 Tranformation of E. coli walk through (Edvotek Inc.)
1. What labels were assigned to the two microcentrifuge tubes?
2. The addition of calcium chloride makes it easier for DNA to cross cell membranes. Why might that be important in this experiment?
3. What material and in what volume was added to the second tube? (Step 7)
4. What term was used to describe adding the cells to a 42 degree C water bath?
5. In Step 16, why do you think the experimenter uses a new inoculating loop for each petri dish?
Discussion Questions
1. What does each surviving bacterial colony on the plates represent?
2. Why did the – pGLO cells grow on the LB plate, but not on the LB + AMP plate?
3. Why were the transformed + pGlO cells able to grow on the LB + AMP plate?
4. What was the purpose of plating – pGLO cells on LB plate (the one with no ampicillin)? Why is this important? What component of the scientific method is this?
5. What was the purpose of plating – pGLO cells on LB + AMP plates? Why is this important?
6. Do you think that the ability of bacteria to acquire DNA from their surroundings is an evolutionary advantage? Write a hypothesis to explain why
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