You are a member of a newly established laboratory in a major health care facility. You are responsible for ensuring that the laboratory maintains an aseptic environment for handling and testing samples as well as ensuring the safety of laboratory personnel. The specific areas you will be in charge of include the following: general cleanliness of the floors/bench tops/refrigerators/incubators, sterilization of glassware and metal tools, maintenance of aseptic conditions while working in the hood/biosafety cabinet, and prevention of contamination by the handling of samples and disposal of biohazardous wastes.
On the basis of the responsibilities listed in this scenario, discuss the factors you would consider in determining how to prevent contamination, control microbial growth, and ensure the safety of samples and laboratory personnel. (See the section General Considerations in Microbial Control in Chapter 19.)
For those cases in which chemical control is appropriate, recommend the best chemicals for use in those situations, taking into account effectiveness, potential adverse effects, and personnel safety.
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