Cell Adhesion Group Activity#1 :
1- What type of analysis led to the prediction that the hypothetical protein TVAG_393390 may function as a protein with cadherin-like functions?
2- List at least 3 predicted protein features of TVAG_393390 that supported the prediction that TVAG_393390 may function as a cadherin-like protein (CLP).
3- What did the predicted topology of CLP and the cellular localization of the GFP-CLP reveal? Why would this result be necessary to function as a cadherin-like protein?
4- What did the adhesion experiments in Fig.5 and Fig.6 reveal about CLP’s function? Utilize the terms heterophilic and homophilic interactions in your explanation.
5- What molecular approach was used to investigate the role of Ca2+ binding for CLP’s function?
6- Based on the findings of the paper, what would you like to investigate next? Describe what method/s you would carry out in the laboratory in order to investigate the biological process that you are interested in researching.
7. If molecular techniques for T.vaginalis are not limited, what is a loss of function experiment you would propose to further investigate your research question of interest stated in Q#6? What readout/result would you assess and what would this experiment help you discover?
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