Procedure I – Net Photosynthesis Rates – 100% Illumination
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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TableTop Science – All Rights Reserved
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LeafSampleTypeIlluminationLevelOxygenConcentrationChange Data (ppm)ElapsedTime(s)OxygenChange Rate(ppm/s)Sun-Adapted100%21.3640.0 (s)Shade-Adapted100%12.9640.0 (s)LeafSampleTypeIlluminationLevelCarbon DioxideConcentrationChange Data(ppm)ElapsedTime(s)Carbon DioxideChange Rate(ppm/s)Sun-Adapted100%-21.3640.0 (s)Shade-Adapted100%-12.9640.0 (s)Observations and Questions
[1] Show your oxygen change rate calculation for the Sun-Adapted data run.
[2] Show your carbon dioxide change rate calculation for the Shade-Adapted data run.
[3] Examine your results. Do your results reveal a possible relationship between (rates of) production of oxygen and carbon dioxide? Explain. Cite your results as part of your explanation.
[4] Using your results for change in oxygen concentration as a reference, discuss whether your Procedure I results confirm research findings about differences in the amount of chlorophyll production for sun-adapted and shade-adapted leaves.
[5] How is the net photosynthesis rate related to the rates you determined? (note that this is not a calculation)
[6] Explain why (or why not) you would expect respiration to occur when the illumination is 100%?
Procedure II – Respiration Rates – 0% Illumination Data Run
Complete the tables below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
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LeafSampleTypeIlluminationLevelOxygenConcentrationChange Data (ppm)ElapsedTime(s)OxygenChange Rate(ppm/s)Sun-Adapted0%-14.0140.0 (s)Shade-Adapted0%-8.4140.0 (s)LeafSampleTypeIlluminationLevelCarbon DioxideConcentrationChange Data (ppm)ElapsedTime(s)Carbon DioxideChange Rate(ppm/s)Sun-Adapted0%14.0140.0 (s)Shade-Adapted0%8.4140.0 (s)Observations and Questions
[7] Sample Calculation: Show your oxygen change rate calculation for the Sun-Adapted data run.
[8] Sample Calculation: Show your carbon dioxide change rate calculation for the Shade-Adapted data run.
[9] Cite your results for the change in carbon dioxide concentration as a reference and compare the respiratory rates for sun-adapted and shade-adapted leaves. Explain the biological principles/bases underlying this comparison.
[10] What would be the photosynthesis rate if you measured it at 0% illumination in Procedure II? Explain your answer. Cite your results from Procedure I to assist with your explanation.
Procedure III – Low-Light Net Photosynthesis Rates – 15% Illumination
Complete the table below using your data and information found under the Background tab (see the Summary of Needed Formulas section)
LeafSampleTypeIlluminationLevelOxygenConcentrationChange Data (ppm)ElapsedTime(s)OxygenChange Rate(ppm/s)Sun-Adapted15%4.7840.0 (s)Shade-Adapted15%8.1940.0 (s)4
TableTop Science – All Rights Reserved
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Observations and Questions
[11] Sample Calculation: Show your oxygen change rate calculation for the Sun-Adapted data run.
[12] Based on the data you collected in Procedures I (100% illumination) and III (15% illumination), which system of leaves produces the most oxygen: Sun-adapted or shade-adapted? Explain how your results support your answer.
[13] Are your results from Procedure II and Procedure III consistent with research findings that there are differences in chlorophyll production and enzyme activity between shade-adapted leaves and sun-adapted leaves? Explain your reasoning. Cite your results from Procedure II and Procedure III to support your explanation.
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