here is what i need the paper to have the protein that i have choose is P53 protein . the task is given and try your best with it and please contact me if you need more info here is what you need to fallow : RESEARCH PROPOSAL General tips A properly prepared research proposal clearly states the objective(s) of your research (poses the
question), provides some background information that explains why your question is important, clearly describes your experimental design (the steps you will follow and methods you will use), and indicates expected outcomes of these steps or experiments based upon your background knowledge and sound biochemical judgment. Background reading can include your textbook, the internet or other sources you can find. An alternative hypothesis will be included because your results may not be as predicted. In the end, a brief summary of follow-up experiments is proposed, should your results not be as expected. Proposal Outline 1. Question: Clearly state a question to which you do not know the answer. The question must be answerable scientifically and, for this particular proposal, must be answerable using one or more biochemical methods. The biochemical methods need not necessarily be the methods we used in class. Your question might or might not have already been answered in a published article. This exercise is not designed to test how well you are acquainted with the scientific literature but how well you can design experiments to clearly answer a question based on your scientific understanding, logic, and scientific originality. Do not plagiarize! Plagiarism is “to use, and pass off as one’s own, someone else’s writing or speech”. A plagiarized report will result in a lower grade for the entire course. 2. Statement of Purpose (Title): The title is put in the form of a statement rather than a question. Thus, question: Is the activity of the enzyme protein phosphatase 1 decreased in cancer cells? Statement (title of proposal): Activity of protein phosphatase 1 in cancer cells. 3. Background Information: Imagine what a reader of your proposal needs to know, in order to understand the context of your question and why it is important. Include all sources you used for your information in parenthesis in the text either by the number the source has in your reference list at the end of the proposal or by the name of the author(s) and the year of publication. 4. Hypothesis: The expected outcome of your proposed experiments. Example: The activity of the enzyme protein phosphatase 1 is decreased in cancer cells. 5. Alternative Hypothesis: A possible different outcome of your proposed experiments. Example: The activity of the enzyme protein phosphatase 1 is unaffected in cancer cells. 6. Experimental Design: Indicate step-by-step specifically what methods you are going to use and what particular results you expect from each step or method based on your hypothesis. 7. Follow-up Experiments: Briefly describe the experiments you think appropriate, if the outcomes of your research are not as expected, for example if your hypothesis were not true. 8. References: List all sources you used in the Background Information part or the Experimental Design part, but include here enough information so that the reader can find the source. for the method you need to use try to choose one of these (Identification of Serum Glycoproteins by SDS-PAGE , Ion Exchange and Size Exclusion Chromatography ,gel filtration, isometric foc )
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