Determining the number of people that are actively infected with a virus often relies on detection of the virus in an individual

1. You are planning to preform an ELISA to detect IFN. Part of this assay requires the assessment of known concentrations of IFN in order to generate a standard curve. You have purchased 1mL of IFN at a concentration of 500g/mL to generate a series of samples with concentrations from 25ng/mL to 0.32pg/mL. Describe how you would perform the dilutions necessary to generate 8 samples of 100L with each
concentration being one fifth the concentration of the previous one. You only have 1.5mL tubes and p20, p200 and p1000 pipets. Show your calculations and remember to include the units.
2. Determining the number of people that are actively infected with a virus often relies on detection of the virus in an individual. This can be difficult, because people have to be tested during an active infection in order to detect virus. In most cases people will mount an antibody response to a virus which can be detected for months to years after the infection. Because of 321 you developed a love of virology and immunology that leads you to go to grad school and study the COVID19 pandemic. Part of your research is to determine the proportion the residents of NYC that were infected with SARS-CoV-2. a. What assay would be the most useful for determining if a person has been infected with SARS-CoV-2. Assume that you have at your disposal a peptide that contains an epitope that is unique to SARS-CoV-2 and universally respondedagainst. Please justify your reasoning. Design an experiment using this assay that would be able to test if a person was infected with SARS-CoV-2. You also have all the reagents and equipment that you used in the lab. Make sure you include all the necessary controls.
 
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