# Effect of Substrate Concentration on Reaction Rate

Description

Pretty straightforward assignment complete whats asked in each question including the plots,etc

The data table below is from an enzyme kinetic assay.Absorbance & Time data for Effect of Substrate Concentration on Reaction Rate

Use the above data to calculate the substrate concentration in each of the samples 1-6. Assume that the totalvolume for each sample is 6.0 mL. Show your calculation below only for sample 1. Do the other calculations, butyou do not need to show the work. Write the concentration values in the red highlight boxes above.
On a piece of graph paper or in excel plot Abs vs time for each data set, and from those plots determineV 0 for each substrate concentration. The extinction coefficient is 1354 M -1 cm -1 and pathlength = 1cm. Show yourwork for one of the V 0 calculations. Convert your V 0 to μM/sec, then f ill in the table below based on yourcalculations for this question. Recall that V 0 means the rate very early. <>Time (Min) Abs ofSample 1Abs ofSample 2Abs ofSample 3Abs ofSample 4Abs ofSample 5Abs ofSample 61.0 mL 0.6M substrate0.5 mL 0.6M substrate0.25 mL 0.6 Msubstrate1.0 mL 0.06M substrate0.5 mL 0.06M substrate0.5 mL 0.06M substrateM[substrate]M[substrate]M[substrate]M[substrate]M[substrate]M[substrate]0 0 0 0 0 0 00.5 0.474 0.462 0.400 0.234 0.154 0.0611.0 0.832 0.813 0.754 0.514 0.365 0.1501.5 1.032 1.036 0.988 0.759 0.571 0.2442.0 1.140 1.163 1.124 0.928 0.752 0.3402.5 1.205 1.216 1.215 1.073 0.889 0.4283.0 1.243 1.266 1.267 1.155 1.013 0.5183.5 1.269 1.288 1.294 1.218 1.108 0.5974.0 1.293 1.305 1.313 1.260 1.176 0.6734.5 1.312 1.313 1.333 1.295 1.221 0.7525.0 1.338 1.323 1.347 1.328 1.264 0.818[Substrate], M V o , μM/sec0 0
Construct a plot of V 0 vs [Substrate]. Use that plot to show Km and Vmax
Construct a plot of . Show on this plot how to determine 1 vs Km and VmaxV 01[Substrate]
Compare the values of Km and Vmax that you determined for questions 3 and 4.Should they be the same/close? If they are not, why not.

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Proceed to pay for the paper so that it can be assigned to one of our expert academic writers. The paper subject is matched with the writer’s area of specialization.
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