Define the following and explain how each relates to the lactase chemical reaction . Be sure to read the information located in the Home, Background and Explore Model tabs to the left of the simulate tab.
a.Enzyme
b.Substrate
c.Active site
d.Rate of reaction
e.Activation Energy
Simulation Option 1: Temperature
Start with the initial lactose at 500 mg/dL and the pH at 7. Do not vary these parameters while testing for temperature, they are environmental controls. The temperature is in degreesCelsius.
Vary the temperature by 20oC (you can slide the cursor over to the right or type the number directly in the box).
Run each experiment 3 times (for reliability in the results.) and record the rate or reaction in the table below. The rate of reaction should be in mg/dL of glucose per minute. Place the arrow on the line in the graph and a “+” symbol appears Click on the line at the end of the 20 minutes and the concentrations will be displayed at the bottom of the graph under the “X” axis (minutes).
0oC
20oC
40oC
60oC
80oC
Trial 1 (final number at 20 minutes/20 = rate per minute)
Trial 2(final number at 20 minutes/20 = rate per minute)
Trial 3(final number at 20 minutes/20 = rate per minute)
Average mg/dl for trials per minute
Questions for Simulation 1
What are the results of the simulation? What is the optimal temperature?
Explain why your results of the optimal temperature of the lactase enzyme make sense for the human enzyme? Explain the results in terms of the structure and function of proteins.
Simulation Option 2: pH
Start with the initial lactose at 500 mg/dL and the temperature at 25 degrees Celsius. Do not vary these parameters while testing for pH, they are environmental controls.
Vary by a pH of 3. Run each experiment 3 times (for reliability in the results.)
Record the rate of reaction in the table below. The rate of reaction should be in mg/dL of glucose per minute (see directions in simulation #1)
pH
1
4
7
10
13
Trial 1 (final number at 20 minutes/20 = rate per minute)
Trial 2 (final number at 20 minutes/20 = rate per minute)
Trial 3 (final number at 20 minutes/20 = rate per minute)
Average mg/dl for trials
Questions for Simulation 2
What are the results of the simulation? What is the optimal pH?
Explain why your results of the optimal pH of the lactase enzyme make sense for the human enzyme? Explain the results in terms of the structure and function of proteins
Analysis Questions:
Write the decomposition chemical reaction for the effect of lactase on lactose. Indicate the substrate, the enzyme, and the products.
Each enzyme operates at an ideal pH and temperature, which may vary for other enzyme.
(a) Pepsin is an enzyme found in the human stomach for digesting protein. What would be the optimum pH conditions for pepsin? What effect does entry into the intestines have on pepsin?
(b) Some bacteria, called thermophiles, live in the hot springs in our Western National Parks. Explain what possible adaptations allow survival under such environmental conditions
Predict how the milk digestion is affected in individuals that lack functional copies of the gene encoding lactase. Justify your response. Why would having the enzyme lactase as an adult be an evolutionary advantage?
Write a summary statement for the biological principles that you have learned from this laboratory exercise.
The post Explore Model tabs to the left of the simulate tab appeared first on Homework Aider.
What Students Are Saying About Us
.......... Customer ID: 12*** | Rating: ⭐⭐⭐⭐⭐"Honestly, I was afraid to send my paper to you, but you proved you are a trustworthy service. My essay was done in less than a day, and I received a brilliant piece. I didn’t even believe it was my essay at first 🙂 Great job, thank you!"
.......... Customer ID: 11***| Rating: ⭐⭐⭐⭐⭐
"This company is the best there is. They saved me so many times, I cannot even keep count. Now I recommend it to all my friends, and none of them have complained about it. The writers here are excellent."
"Order a custom Paper on Similar Assignment at essayfount.com! No Plagiarism! Enjoy 20% Discount!"
