Homework 8

When the short tandem repeat (STR) known as CSF1PO was amplified via PCR, the following allele lengths were determined:

allele	length (bp)
6	321
7	325
12	?

Based on the other data in the table, what would you expect the missing length value for allele 12 to be?

Question 1 options:

329 bp

345 bp

4 bp

646 bp

Table of Contents

Question 2 (10 points)

In the DNA sequences shown below, which choice(s) highlight(s) the best PCR primer target sites that could be used to detect polymorphisms in the length of the simple sequence repeat region in different individuals?

Question 2 options:

sequence (a)

sequence (b)

sequence (c)

both sequence (a) and sequence (b)

Question 3 (10 points)

Explain why the overwhelming majority of single nucleotide polymorphisms only have two alleles.

Question 4 (10 points)

Is GWAS useful for helping determine why some of us have blue eyes while others do not? Explain why or why not.

Question 5 (10 points)

In a certain population that is in Hardy-Weinberg equilibrium, the frequency of homogyzous recessive individuals is 1%. Determine the frequency of the:

1. recessive allele

2. dominant allele

3. heterozygous phenotype

4. homozygous dominant phenotype

Question 6 (10 points)

1,200 people were sampled from a population that is in Hardy-Weinberg equilibrium. Initial genotypic results revealed that there were 432 homozygous dominant individuals. Given this data, what are the numbers of Aa and aa individuals? NOTE: You must show and clearly label all work to receive credit.

Question 7 (10 points)

Stickleback fish that are deep in the ocean can experience a mutation wherein they experience a “loss-of-function” of their spikes. Heterozygotes actually look the same as homozygous wild types at this depth. If a homozygous deep-water wild type stickleback mated with a homozygous shallow-water wild type, what phenotypes would you expect to see?

Question 8 (10 points)

Discuss the characteristics of a “model organism” used in genetics. What makes something a “model organism” and thus well-suited for genetics research?

Which model organism discussed in the course materials is used to study genes related to cell division?

Question 9 options:

	Arabidopsis
	yeast
	C. elegans
	zebrafish

Question 10 (10 points)

p53 is a very versatile gene that controls many tumor-suppressing functions. What are some of the specific functions it controls, and how is it possible for it to exert so much influence over so many different biochemical pathways?

NOTE: All answers must be detailed and fully address the questions asked, and must incorporate and reference, at minimum, the course materials. Brief and/or insufficient answers that do not reflect rigorous academic effort and college-level critical thought on the part of the student will receive little to no credit.

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Homework 8

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