Review Ch. 3 of Microbiology.
Review “Staining Bacteria: The Gram Stain” simulation (http://higheredbcs.wiley.com/legacy/college/black/0471420840/animations/ch03/ch3Staining_Bacteria/index.html).
This simulation is also found on the student website.
Complete the open book quiz. The quiz will be provided by your instructor.
SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each)
Check or underline the correct answer
Section: Multiple Choice
1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids?
Gram stain
Schaeffer-Fulton stain
Acid-fast stain
Lipid stain
Spore Stain
2. Which of the following stains is used to classify microorganisms based on their cell wall content?
Capsular stain
Gram stain
Spore stain
Negative stain
Methylene blue
3. In the Gram stain, Gram-positive bacteria stain with a _________ color due to the incorporation of the _________ dye into their cell wall.
purple, safranin
purple, crystal violet
red, safranin
red, crystal violet
4. What is the function of the mordant iodine in a Gram staining procedure?
A mordant causes the crystal violet to run.
A mordant fixes the bacteria to the slide.
A mordant keeps dye attached to an object.
All of the above
5. What is the order of reagents used in the Gram stain?
crystal violet, iodine, safranin, alcohol
alcohol, crystal violet, iodine, safranin
iodine, crystal violet, safranin, alcohol
crystal violet, iodine, alcohol, safranin
crystal violet, safranin, alcohol, iodine
6. The presence of a capsule around bacterial cells usually indicates their increased disease-causing potential and resistance to disinfection. Capsules are generally viewed by ________
spore staining.
scanning electron microscopy.
Gram staining
Negative stain
Section: True or False
7. Fluorescent microscopes use an ultraviolet (UV) light source and fluorochromes as stains.
True
False
8. Older, pure cultures of Gram-positive organisms can appear red when Gram stained.
True
False
Section: Matching
9. Use of two or more dyes to differentiate among bacterial species or to distinguish various structures of an organism; for example, the Gram stain.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
10. A technique for observing flagella by coating the surfaces of flagella with a dye or a metal such as silver.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stai
Cationic dye (also called basic dye)
11. Technique of staining the background around a specimen, leaving the specimen clear and unstained.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
12. A differential stain used to make endospores easier to visualize.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
13. A single dye used to reveal basic cell shapes and arrangements.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
14. A differential stain for organisms that are not decolorized in alcohol, such as the bacteria that cause Hansen’s Disease (leprosy) and tuberculosis.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
15. An ionic compound, used for staining bacteria, in which the positive ion imparts the color.
Differential stain
Flagellar stain
Gram stain
Negative stain
Schaeffer-Fulton spore stain
Simple stain
Ziehl-Neelsen acid-fast stain
Cationic dye (also called basic dye)
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