Title: Characterization of Dendrimer: A potent siRNA delivery agent to the primary cells
Write an introduction for a research paper Titled “Characterization of Dendrimer: A potent siRNA delivery agent to the primary cells”. (Title may be changed; you can give a new one)
Write it within 400 words which should include the following sections
1. RNA interference: targeted gene silencing (1-2 sentences)
2. difficulties to deliver the siRNA (or other genetic materials like DNA): Main problem is targeted delivery, endosomal escape (find more reasons)
3. Importance of delivery agent to deliver siRNA, from here the dendrimer story begins.
4. Dendrimer as a potent delivery agent (reference papers added)
5. Tell about Dendrimer (mainly use the reference papers as a source) the possible side effect of Dendrimer needs to be explored should be explored
6. About Antigen presenting cells (Macrophages and Dendritic cells specially)
7. Why we choose Macrophages and Dendritic cells as a model system to evaluate toxicity (The argument we have is: phagocytic capacity of these cells leads to accumulation of nanoparticles in these cells, that’s why it’s important to check the functionality of these cells loaded with Dendrimer
8. Write about Immunometabolism and how Immunometabolism governs immune cells function and possibility of nanoparticle mediated dysfunction of metabolism in macrophages and dendritic cell polarization (when stimulated by LPS/IL-4 or remain untreated)
The above mentioned points should be in the introduction of the paper additionally mention about relation of Dendrimer with the different parameters measured to prepare the papers.
*** Metabolism is main focus so put stress on Immunometabolism
The experiments we did to prepare the paper is
1. Phagocytosis assay (showing the phagocytic capacity of the cells)
2. Cell activation (CD86/MHC-II) as a cell activation marker
3. Antigen presentation assay (using a OTII mice model, co culture of T cells with gold loaded Dendritic cells) (production of IL-4, IFN-y-IL-17 by activated T cells)
4. Cytokine immunoassays (checked IL-6, IL-12p70, MCP-1, TNF-α, IL-10, and IFNγ secretion)
5. NO production
6. Reactive oxygen species Production
7. Metabolic flux analysis (Mito and Glycostress assay using Seahorse XF analyzer)
8. Also the western blot (we showed the efficient delivery of siRNA and knockdown of targeted protein)
I will upload files to help you out with Immunometabolism but for the rest of the parameters rest you have to find from different articles.
For source please use diffrent research articles and review papers.
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